Open Access
Table 1.
Primers used for detection of trypanosomes.
| Primers | Sequences | Trypanosomes | Size (bp) |
|---|---|---|---|
| ITS 1 CF | 5′- CCGGAAGTTCACCGATATTG -3′ | T. congolense | 620–710 |
| ITS1 BR | 5′- TTGCTGCGTTCTTCAACGAA -3′ | T. brucei b. | 480 |
| T. evansi | 480 | ||
| T. vivax | 250 | ||
| T. simiae | 400 | ||
| TCF 1 | 5′-GGACACGCCAGAAGGTACTT-3′ | T. congolense forest | 350 |
| TCF 2 | 5′-GTTCTCGCACCAAATCCAAC-3′ | ||
| TCS 1 | 5′-CGAGCGAGAACGGGCAC-3′ | T. congolense savannah | 316 |
| TCS 2 | 5′-GGGACAAACAAATCCCGC-3′ | ||
| TCK 1 | 5′-GTGCCCAAATTTGAAGTGAT-3′ | T. congolense kilifi | 294 |
| TCK 2 | 5′-ACTCAAAATCGTGCACCTCG-3′ |
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