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Amplicon products on agar gel electrophoresis. Lane 1, positive control of Dirofilaria immitis; lanes 2–4, samples from the worm removed from the patient and amplified by D. immitis specific primers; lane 5, negative control; lane 6, molecular mass marker; lanes 7–9, samples from the worm removed from the patient and amplified by Dirofilaria repens specific primers; and lane 10, D. repens positive control.
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