Table 1.
Cytokine production in serum samples or splenocyte cultures from immunised BALB/c mice.
| Groups (n = 10) | Production of cytokine |
|||
|---|---|---|---|---|
| IFN-γ | IL-2 | IL-4 | IL-10 | |
| splenocyte cultures (pg/mL) | ||||
| PBS | 69.590 ± 5.43 | 861.23 ± 20.51 | 159.11 ± 33.21 | 133.88 ± 14.92 |
| 10 μg rTgPGAM 2 | 79.40 ± 8.14 | 976.28 ± 79.78 | 171.29 ± 26.68 | 134.17 ± 9.23 |
| 20 μg rTgPGAM 2 | 109.69 ± 3.47☆ | 1050.78 ± 86.57☆ | 181.67 ± 34.34 | 136.49 ± 18.46 |
| 30 μg rTgPGAM 2 | 187.01 ± 6.43▲ | 1451.35 ± 21.25▲ | 219.35 ± 30.26☆ | 137.28 ± 8.78 |
| 40 μg rTgPGAM 2 | 170.95 ± 9.21▲ | 1453.17 ± 99.43▲ | 214.81 ± 15.31☆ | 137.42 ± 10.39 |
| serum samples (ng/mL) | ||||
| PBS | 16.34 ± 2.84 | 27.03 ± 1.45 | 25.96 ± 1.84 | 35.29 ± 2.42 |
| 10 μg rTgPGAM 2 | 18.56 ± 1.28 | 27.56 ± 3.48 | 26.33 ± 1.74 | 37.68 ± 4.81 |
| 20 μg rTgPGAM 2 | 21.75 ± 1.50 | 29.02 ± 2.74 | 27.51 ± 2.89 | 38.34 ± 6.20 |
| 30 μg rTgPGAM 2 | 38.65 ± 3.43☆ | 46.94 ± 7.08☆ | 39.01 ± 2.84☆ | 38.74 ± 5.85 |
| 40 μg rTgPGAM 2 | 35.12 ± 3.98☆ | 43.02 ± 2.73☆ | 37.09 ± 3.01☆ | 39.49 ± 5.11 |
The results are presented as the arithmetic means ± standard errors of three replicate experiments. The values for IFN-γ are for 96 h. The values for IL-2 and IL-4 are for 24 h, and the values for IL-10 are for 72 h. ☆P < 0.05, ▲P < 0.01 (vaccinated vs. PBS group).
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