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Table 1.

Cytokine production in serum samples or splenocyte cultures from immunised BALB/c mice.

Groups (n = 10) Production of cytokine
IFN-γ IL-2 IL-4 IL-10
splenocyte cultures (pg/mL)
PBS 69.590 ± 5.43 861.23 ± 20.51 159.11 ± 33.21 133.88 ± 14.92
10 μg rTgPGAM 2 79.40 ± 8.14 976.28 ± 79.78 171.29 ± 26.68 134.17 ± 9.23
20 μg rTgPGAM 2 109.69 ± 3.47 1050.78 ± 86.57 181.67 ± 34.34 136.49 ± 18.46
30 μg rTgPGAM 2 187.01 ± 6.43▲ 1451.35 ± 21.25▲ 219.35 ± 30.26 137.28 ± 8.78
40 μg rTgPGAM 2 170.95 ± 9.21▲ 1453.17 ± 99.43▲ 214.81 ± 15.31 137.42 ± 10.39
serum samples (ng/mL)
PBS 16.34 ± 2.84 27.03 ± 1.45 25.96 ± 1.84 35.29 ± 2.42
10 μg rTgPGAM 2 18.56 ± 1.28 27.56 ± 3.48 26.33 ± 1.74 37.68 ± 4.81
20 μg rTgPGAM 2 21.75 ± 1.50 29.02 ± 2.74 27.51 ± 2.89 38.34 ± 6.20
30 μg rTgPGAM 2 38.65 ± 3.43 46.94 ± 7.08 39.01 ± 2.84 38.74 ± 5.85
40 μg rTgPGAM 2 35.12 ± 3.98 43.02 ± 2.73 37.09 ± 3.01 39.49 ± 5.11

The results are presented as the arithmetic means ± standard errors of three replicate experiments. The values for IFN-γ are for 96 h. The values for IL-2 and IL-4 are for 24 h, and the values for IL-10 are for 72 h. P < 0.05, ▲P < 0.01 (vaccinated vs. PBS group).

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