Determination of specific anti-rTgPGAM 2 humoral response in BALB/c mice. Two weeks after the immunisation schedule was completed, blood samples were obtained and antibody titres were determined by ELISA with rTgPGAM 2 as the bound target. The specific total IgG (A), IgG isotype (B) and IgA titres (C) in sera from mice vaccinated with different dosages of rTgPGAM 2 or PBS as a control were determined in duplicate. The results are expressed as the mean of the OD₄₉₂ ± SD (n = 10) and are representative of three experiments. *P < 0.05, **P < 0.01 (vaccinated vs. PBS group).