Morphological images of female adults of the Dirofilaria species isolated from the present case (left column, A–E) and Dirofilaria immitis (right column, F–J). A, F: direct images of the cephalic parts under an optical microscope. B, G: direct images of the caudal parts under an optical microscope. C, H: cross-sectional tissue sections (hematoxylin and eosin stain). D, I: low-magnification images of the body surfaces under a scanning electron microscope (SEM). E, J: high-magnification images of the body surfaces under SEM. Scales bars: A, B, C, F, G, 100 μm; H, 200 μm. CL, Cuticular Layer; ELR, External Longitudinal Ridge; I, Intestine; ML, Muscular Layer; U, Uterus.