Alignment of the deduced amino acid sequence of GlyR from R. microplus (KJ476181), GlyR from C. Intestinalis (BAI66458), GlyRa (NP_000162) and GlyRb (NP_000815) from H. sapiens, GlyR from B. taurus (NP_776746), and GlyR from D. rerio (NP_571477). The cysteines forming the Cys-loop are highlighted by red shading and the putative membrane domains (TM1–TM4) are highlighted by gray shading. The PAR residues are highlighted in yellow shading. The glycine residue marked in turquoise likely confers upon the receptor high sensitivity to ivermectin. The approximate locations of the glycine binding domains are labeled as A-F. Known residues in human GlyRa that play a role in glycine binding are highlighted in green [22].