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Table 1.
Summary of data extracted from articles which met the selection criteria.
| Reference | Cell line | T. cruzi strain (DTU) | MOI | Incubation time | Medium (Supplement.) | Results | Type of study |
|---|---|---|---|---|---|---|---|
| Osuna et al., 1990 [19] | HeLa | Human isolate (NT) | 1, 5 & 10 | 2 h | MEM (ND) | % of infection % PI 2 h MOI 1 (21.9 & ND) MOI 5 (35.5 & 1.6) MOI 10 (20.2 & 2.2) | Biological: biochemistry |
| Faria et al., 2008 [8] | HeLa | Y (TcII) | 40 | 0, 1, 3, 6, 12 & 24 h | DMEM (10% FCS) | % of infection at hrs: 1 (>20%), 3 (>40%), 6 (<50%), 12 (>50%) & 24 (>80%) | Biological: biochemistry |
| Sartori et al., 2003 [26] | HEp-2 | Tulahuen (TcVI) | 10 | 2 h | MEM (10% FCS) | % of infection 60%–80% | Biological: infectivity |
| Morris et al., 1988 [16] | HUVEC | Tulahuen (TcVI) | 1.5–2 | 4 d | M199 (20% FCS 10% HS) | % of infection at 4 days: 40–50%. In 75% of the cells NoA of at least 5 | Biological: biochemistry |
| Wittner et al., 1995 [36] | HUVEC & HUSMC | Tulahuen (TcVI) | 1.5 & 2 | 72 h | ND (ND) | % of infection at hrs: 1 (<1%), 6 (<1%), 24 (10%), 48 (20–40%) & 72 (>80%) | Biological: biochemistry |
| Todorov et al., 2003 [30] | HUVEC | Dm28c (TcI) | 1 & 4 | 3 h – wash & – 3 d | M199 (10% FCS) | NoA/100 cells at 3 hrs < 6. NoA/100 cells at 3d 298.4 | Biological: infectivity |
| Mukherjee et al., 2004 [17] | HUVEC & HUSMC | Tulahuen (TcVI) | 1.5 & 2 | 48 h – wash – 24 h | DMEM (20% FCS 5% HS) | % of infection at hrs: 24 (20%) 48 (50%) & 72 (80%) | Biological: biochemistry |
| Hassan et al., 2006 [12] | HUVEC & HUSMC | Tulahuen & Brazil (TcVI & NT) | 1.5 & 2 | 48 h – wash – 24 h | ND (ND) | % of infection at hrs: 24 (20%), 48 (50%) & 72 (80%) | Biological: biochemistry |
| Soares et al., 2011 [27] | PBMCs | Y (TcII) | 10 | 3 h – wash & add compound – 24 h | RPMI (10% FCS) | Mean % of infection & NoA/infected cell from 8 human donors: 32% ± 21,48% & 2,61 ± 1,61 | Pharmacological |
| Souza et al., 2007 [28] | PBMCs | Y (TcII) | 10 | 3 h | RPMI (ND) | % of infection at 3 h: monocytes (80%), T-lymphocytes (1%) & B-lymphocytes (5%) | Immunological |
| Williams and Remington, 1977 [35] | PBMCs | Tulahuen (TcVI) | 1 | 2, 24, 48 & 72 h | M199 (40% HS) | % of infection monocytes: hrs 2 (29%), 24 (25%) & 48 (26%). Macrophages: average % of infection 90% at all time points. | Biological: infectivity |
| Coelho dos Santos et al., 2010 [2] | PBMCs | Y (TcII) | 5 | 3, 48 & 96 h | RPMI (10% FCS) | % of infection at hrs: 3 (>50%), 48 (25–50%) & 96 (25–50%) | Immunological |
| Piras et al., 1982 [21] | Vero | EP, BEC, MEN (NT, NT & NT) | NS | 2 h | MEM (10% FCS) | Ii: EP strain 0.8; BEC strain 0.19. | Biological: morphology |
| Piras et al., 1987 [20] | Vero | EP (NT) | ND | 2 h | MEM (10% FCS) | Ii: medium alone 0.4, with FCS 1.0 | Biological: infectivity |
| Urbina et al., 1988 [31] | Vero | EP (NT) | 20 | 2 h – wash – 100 to 180 h | MEM (10% FCS) | % of infection at hrs 2 (65%) 24 (69%), 48 (70%), 72 (70%), 96 (85%). Mean NoA/cell: 60 at 96 h | Pharmacological |
| Urbina et al., 2002 [32] | Vero | EP (NT) | 10 | 2 h – wash – 96 h | MEM (1% FCS) | % of infection & NoA/cell at 96 h: 23% & 30 | Pharmacological |
| Revollo et al., 1998 [24] | Vero | Group 19/20: SP104 cl1, Cutia cl4, Gamba, 13379 cl7, OPS21 cl11, SO34 cl4, Cuica cl1, P/209 cl1, Esquilo cl1 & P/11 cl2 (TcI) |
16 | 15–30 h | RPMI (5% FCS) | % of infection & NoA Group 19/20: 86,63 ± 7,89 & 17,98 ± 3,02 | Biological: biochemistry |
| Group 32: MAS1 cl1, CBB cl3, Tu18 cl2, IVV cl4 & MVB cl8 (TcV) |
% of infection & NoA Group 32: 71,46 ± 7,25 & 13,67 ± 2,11 | ||||||
| Group 39:SC43 cl1, Bug2148 cl1, Bug2149 cl1 & SO3cl5 (TcII) |
% of infection & NoA Group 39: 51,27 ± 8,56 & 10,6 ± 2,37 | ||||||
| Pires et al., 2008 [22] | Vero | CL Brener, Tulahuén, JG & Col1.7G2 (TcVI, TcVI, TcII & TcI) |
10 | 18 h – change medium – 6 d | LIT (ND) | % of infection & NoA/infected cell at 6d: TulaWT (wild-type) 7% & 25, TulaRFP1 9% & 20, TulaGFP2 7% & 30. | Biological: infectivity |
| Vilchez-Larrea et al., 2012 [34] | Vero | CL Brener (TcVI) | 50 | 24 h – change medium – 5 d | DMEM (10% FCS) | % of infection & NoA: days 2 (37.20% & < 1), 4 (20.81% & < 3), 6 (27.36% & < 4) | Pharmacological |
d: Days; DTU: Discrete typing unit; Ii:Infection index; FCS: Fetal calf serum; h: Hours; HS: Human serum; Ii: Infection index; MOI: Multiplicity of infection; PI: Parasite index; ND: No data; NoA: Number of amastigotes; NT: Not typified; PBMCs: Peripheral blood mononuclear cells; Tc: Trypanosoma cruzi DTU.
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