Open Access
Table 1.
Primer sequences for PCR detection of five human malaria parasites
| Plasmodium spp | Size (bp) | Primers | Primer sequences (5′→3′) | |
|---|---|---|---|---|
| Nested 1st round primers | Genus-specific | 1600–1700 | rPLU1 | TCAAAGATTAAGCCATGCAAGTGA |
| rPLU5 | CCTGTTGTTGCCTTAAACTTC | |||
| Nested 2nd round primers | Genus-specific | 235 | rPLU3 | TTTTTATAAGGATAACTACGGAAAAGCTGT |
| rPLU4 | TACCCGTCATAGCCATGTTAGGCCAATACC | |||
| Nested 2nd round species-specific primers | P. vivax | 121 | rVIV1 | CGCTTCTAGCTTAATCCACATAACTGATAC |
| rVIV2 | ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA | |||
| P. falciparum | 206 | rFAL1 | TTAAACTGGTTTGGGAAAACCAAATATATT | |
| rFAL2 | ACACAATGAACTCAATCATGACTACCCGTC | |||
| P. ovale | 226 | rOVA1 | ATCTCTTTTGCTATTTTTTAGTATTGGAGA | |
| rPLU2 | ATCTAAGAATTTCACCTCTGACATCTG | |||
| P. malariae | 145 | rMAL1 | ATAACATAGTTGTACGTTAAGAATAACCCC | |
| rMAL2 | AAAATTCCCATGCATAAAAATTATACAAA | |||
| P. knowlesi | 153 | Pmk8 | GTTAGCGAGAGCCACAAAAAAGCGAAT | |
| Pmkr9 | ACTCAAAGTAACAAAATCTTCCGTA | |||
| Single-Step PCR primers | P. knowlesi | 200 | Pkr140-5F | CAGAGATCCGTTCTCATGATTTCCATGG |
| Pkr140-5R | CTRAACACCTCATGTCGTGGTAG |
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