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Specificity of PfHsp70-x antiserum: (A) Total lysates from normal and infected erythrocytes analysed by immunoblotting with normal mouse serum (left) and PfHsp70-x antiserum (right). (B) Two-dimensional electrophoresis performed using lysate from parasite infected erythrocytes and probed with PfHsp70-x antiserum. (C) Lysates from infected erythrocytes analysed by immunoblotting with other host and parasite heat shock protein antisera. Lane 1: probed with PfHsp70-x antiserum, Lane 2: probed with PfHsp70-1 antiserum, Lane 3: probed with Host Hsp70 antiserum, Lane 4: probed with both PfHsp70-x and PfHsp70-1 antiserum and Lane 5: probed with both PfHsp70-x and Host Hsp70 antiserum. The blots indicate that PfHsp70-x antiserum is specific and does not cross-react with any other host or parasite chaperone.
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