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Re-annotation of PfHsp70-x sequence: (A) Original nucleotide sequence – intron is shown in lower case with splice sites in italics. ATG represents the start site, TAA is the in-frame stop codon created upon splicing and t is the erroneously designated SNP in the intron. (B) New nucleotide sequence – atg represents the alternate start site annotated following t > a change in the nucleotide sequence. Now the gene lacks intron and encodes the entire ORF. Sequence in lower case before atg is now a part of 5′ UTR whereas the sequence after atg is part of the ORF. (C) Original protein sequence – * represents the in-frame stop codon which resulted in a truncated polypeptide. (D) New protein sequence – the sequence in blue is the new N-terminal sequence and underlined region represents the putative signal peptide. (E) Genomic location of PfHsp70-x.
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